mucin 2  (Bioss)

Journal: Drug Metabolism and Disposition

Article Title: Canine duodenal organoids as a functional platform for intestinal CYP regulation and drug metabolism studies

doi: 10.1016/j.dmd.2025.100191

Figure Lengend Snippet: Expression of differentiation markers and CYP genes in duodenal organoids under differentiation conditions. Five differentiation markers, alkaline phosphatase ( ALP ), CgA , leucine-rich repeat-containing G-protein coupled receptor 5 ( Lgr5 ), lysozyme ( Lyz ), and mucin 2 ( MUC2 ), as well as 2 CYP genes, CYP2B11 and CYP3A98 mRNA, were quantified using RT-qPCR. Two biological replicates (dog 1 and dog 3) were used for this analysis, and differentiation/nondifferentiation treatment was replicated 3 times. The error bars represent the SD. ∗ P < .05; ∗∗ P < .01. NS, not significant.

Article Snippet: We quantified a variety of lineage-specific marker genes, including alkaline phosphatase for epithelial cells, CgA for enteroendocrine cells, leucine-rich repeat-containing G-protein coupled receptor 5 ( Lgr5 ) for stem cells, lysozyme ( Lyz ) for Paneth cells, and mucin 2 ( MUC2 ) for goblet cells, as well as drug-responsive genes ( CYP2B11 , CYP3A98 , CAR , and PXR ), using RT-qPCR on days 4, 6, and 8 with SYBR Green (Thermo Fisher Scientific) and a CFX96 system (Bio-Rad) following established protocols., , , , , , , GAPDH , HMBS , and SDHA served as reference genes.

Techniques: Expressing, Quantitative RT-PCR

Journal: Animal Nutrition

Article Title: Terpine-4-ol alleviates lipopolysaccharide-induced intestinal injury in weaned piglets by regulating intestinal epithelial cell homeostasis via the Wnt/β-catenin signaling pathway

doi: 10.1016/j.aninu.2024.12.010

Figure Lengend Snippet: Effects of terpine-4-ol on the number of goblet cells, the protein expression of mucin 2 (Muc2) and chromogranin A (ChgA) in the small intestine of immune-stressed piglets. The goblet cells in the jejunum and ileum (A, B). The immunohistochemical staining of Muc2 in the jejunum and ileum (C). The immunohistochemical staining of ChgA in the jejunum and ileum (D). CON, control group; LPS, lipopolysaccharide challenged group; LTP, low dose of terpine-4-ol supplemented group; MTP, middle dose of terpine-4-ol supplemented group; HTP, high dose of terpine-4-ol supplemented group. Different small letters above data columns indicate significant differences among different groups ( P < 0.05).

Article Snippet: The sections were incubated with the first primary antibodies of antigen Ki-67 (Ki-67) polyclonal antibody (1:400, PA5-19462, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), chromogranin A antibody (1:2, ab53464, Abcam, Cambridge, UK), mucin 2 (Muc2) polyclonal antibody (1:1500, 27675-1-AP, Proteintech Group, Rosemont, IL, USA), villin polyclonal antibody (1:100, ab233155, Abcam, Cambridge, UK), cleaved caspase 3 antibody (1:2200, 9664S, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature, respectively.

Techniques: Expressing, Immunohistochemical staining, Staining, Control